BREWING ENZYMES - GLUCOSYLASE

GLUCOSYLASE is an exo-1,4-alpha-D-glucosidase (amyloglucosidase EC.3.2.1.3.) from Aspergillus niger which catalyzes the hydrolysis of the alpha-1,4 and alpha-1,6 linkages of starch producing glucose. During hydrolysis, glucose units are removed in a stepwise manner from the non-reducing end of amylose or amylopectin chains. The rate of hydrolysis depends on the type of linkage as well as on the chain length: alpha-1,4 linkages are more readily hydrolyzed than alpha-1,6 linkages. PURPOSES Production of glucose syrups. Increase of wort fermentability (apparent attenuation limit); some 25% of the total carbohydrate of wort is unfermentable. The addition of Glucosylase to the fermenter converts the unfermentable dextrins to glucose which in turn is fermented to alcohol giving an apparent attenuation higher than 100% without loss of palate fullness or head retention. At the same alcohol concentration as a normal attenuated beer the super-attenuated beer will have required 25% less of...