qPCR - RTqPCR

Your research projects require: - Quantifying gene expression - Detecting and estimating the relative quantity of a pathogen, a virus... - Genotyping from SNPs - Detecting and determining the copy number of a gene - Monitoring the quality of your co-cultures and validating a production test. Real-time PCR or quantitative PCR (qPCR) is one of the most suitable techniques to meet these needs: derived from PCR, it allows for the detection, characterization, and quantification of nucleic acids for numerous applications. qPCR is typically performed using DNA, but for certain specific applications such as gene expression, RNA will be used as the starting material, which is referred to as RT-qPCR (Reverse Transcription quantitative PCR). More specifically, real-time PCR differs from conventional PCR by the use of a labeling system that allows tracking the progress of the reaction. The data collected at each cycle...